Meningococ Si Gonococ Lp UTM

7/25/2019 Meningococ Si Gonococ Lp UTM

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Genul Neisseria

Diagnosticul de laborator in infectii produse deNeisseria gonorrhoeae

Diagnosticul de laborator in infectii produse deNeisseria meningitidis

1)Neisseria gonorrhoeae

Produs patologic:

secreie uretral, secreie glande Bartholini, secreie vaginal, secreie prostatic

la femei se recolteaz n timpul sau la sfrsitul ciclului menstrual

secreie faringian

urin

secreie conjunctival

2)Neisseria meningitidis

Produs patologic:

LCR

secreie faringian

snge

Rezistena: foarte sensibili la temperatura camerei, la uscciune ,recoltarea se face la

patul bolnavului

1. Examenul microscopic, frotiu din produsul patologic

N. gonorrhoeae

o coci !ram"#$ sub form de boab de cafea dispusi diplo

o n infecii acute se gsesc intracelular

o n infecii cronice se gsesc intra si e%tracelular&

'


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Slide 1

Neisseria gonorrhoeae

(Enlarged view)

Slide 2

Nongonoccal urethritis

(Enlarged view)

Slide 3

Chlamydia trachomatis

(Enlarged view)

These images are from the original published atlas: Tenover, F. C., and J. V. Hirschmann. !!".

#nterpretation of $ram stains and other common microbiologic slide preparations. The %pJohn Company,

&alama'oo, (ich. )ermission granted to the *+( (icrobeibrary by )fi'er #nc.

Neisseria Gonorrhoeae

(
http://lib.jiangnan.edu.cn/ASM/029-1.jpghttp://lib.jiangnan.edu.cn/ASM/029-1.jpghttp://lib.jiangnan.edu.cn/ASM/029-2.jpghttp://lib.jiangnan.edu.cn/ASM/029-2.jpghttp://lib.jiangnan.edu.cn/ASM/029-3.jpghttp://lib.jiangnan.edu.cn/ASM/029-3.jpghttp://lib.jiangnan.edu.cn/ASM/029-1.jpghttp://lib.jiangnan.edu.cn/ASM/029-1.jpghttp://lib.jiangnan.edu.cn/ASM/029-2.jpghttp://lib.jiangnan.edu.cn/ASM/029-2.jpghttp://lib.jiangnan.edu.cn/ASM/029-3.jpghttp://lib.jiangnan.edu.cn/ASM/029-3.jpg


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N. meningitidis

o coci !ram"#$ sub form de boab de cafea dispusi diplo

o ncapsulai

Neisseria meningitidis Gramstain of a pure culture. Note t!e c!aracteristic "diplococcal"

arrangement of cells.

textbookofbacteriology.net/.../meningitis.html

meningococ

)
http://textbookofbacteriology.net/themicrobialworld/meningitis.htmlhttp://www.dstl.gov.uk/science_spot/acolyte.phphttp://textbookofbacteriology.net/themicrobialworld/meningitis.html


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#. $zolarea "sunt germeni foarte preteniosi$

se nsmneaz imediat dup recoltare

se nclzeste mediul la )*+C mediile de cultur utilizate !eloz#chocolat, -haier#.artin

Neisseria gonorrhoeae (ATCC43069) colonies growing on Thayer Martin Modi!ied

Agar (Cat. no. "30). #nc$%ated in C&'!or 4 ho$rs at 3 deg. C.

$ncubarea se face cu % obligatoriu 1'( #))* ore la +'%.

/


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+. $dentificarea

%aractere de cultur-

col 012 friabile, asemntoare perlelor&

ncadrarea /n genul Neisseria:

-estul o%idazei este pozitiv 3 4eisseria

5


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Reactia catalazei este pozitiva

Colonia testa pe o lama cu o picatura de )6 apa o%igenata

Reactie pozitiva 3 apar bule

7


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8ermentarea zaharurilor

Gonococ

!L9C:;< =

.>L-:;<

0eningococ

!L9C:;< =

.>L-:;< =

ncadrarea /n specie:

-estul de umflare a capsulei

4 meningitidis pt stabilirea erogrupului

*


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). ntibiograma se face pe medii speciale, conform CL1?&

2. Diagnostic rapid

bazat pe reacii >g#>c se pun n eviden antigenele meningococice direct din LCR

o Reacii de precipitare inelar

o Reacii de aglutinare&

Modul de desfasurare a lucrarii practice

studentii primesc manuscrisul cu Diagnosticul de laborator in infectii produse de

Neisseria gonorrhoeae.Diagnosticul de laborator in infectii produse deNeisseria

meningitidis

Lp se desfasoara interactiv- se discuta pe marginea manuscrisului primit

anterior LP-ului

'& Fiecare student prinde imaginea la microscop si examineaza frotiuri :coci

Gram negativi reniformi in diplo

Bibliografie

Bacteriologie Medicala, Olga Dorobat, editura UTM, 2006

Clinical Microbiology Procedures Handbook, Henry D. Isenberg, editata American Society for

Microbiology, 2004

Koneman's Color Atlas and Textbook of Diagnostic Microbiology,Washington C

Winn, Stephen D. Allen, Gail L. Woods,William M. Janda, Paul C. Schreckenberger, editura Lippincott

Williams & Wilkins, 2005

Manual of Clinical Microbiology , Patrick R. Murray,

Visit Amazon's Patrick R. Murray Page

search results

Learn about Author Central

@
http://shopping.yahoo.com/search;_ylt=AmwIkvVSDn3oMrp235b4BMACyNsA?p=Henry+D.+Isenberghttp://shopping.yahoo.com/search;_ylt=AkzkLWrc9z31QkUKO_a6ltICyNsA?p=American+Society+for+Microbiologyhttp://shopping.yahoo.com/search;_ylt=AkzkLWrc9z31QkUKO_a6ltICyNsA?p=American+Society+for+Microbiologyhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Washington+C+Winnhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Washington+C+Winnhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Washington+C+Winnhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Stephen+D.+Allenhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Gail+L.+Woodshttp://search.barnesandnoble.com/booksearch/results.asp?ATH=William+M.+Jandahttp://search.barnesandnoble.com/booksearch/results.asp?ATH=William+M.+Jandahttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Paul+C.+Schreckenbergerhttp://www.amazon.com/Patrick-R.-Murray/e/B001IGOFSS/ref=ntt_athr_dp_pel_1http://www.amazon.com/Patrick-R.-Murray/e/B001IGOFSS/ref=ntt_athr_dp_pel_pop_1http://www.amazon.com/s/ref=ntt_athr_dp_sr_pop_1?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Patrick%20R.%20Murrayhttp://authorcentral.amazon.com/gp/landing/ref=ntt_atc_dp_pel_1http://shopping.yahoo.com/search;_ylt=AmwIkvVSDn3oMrp235b4BMACyNsA?p=Henry+D.+Isenberghttp://shopping.yahoo.com/search;_ylt=AkzkLWrc9z31QkUKO_a6ltICyNsA?p=American+Society+for+Microbiologyhttp://shopping.yahoo.com/search;_ylt=AkzkLWrc9z31QkUKO_a6ltICyNsA?p=American+Society+for+Microbiologyhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Washington+C+Winnhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Washington+C+Winnhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Stephen+D.+Allenhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Gail+L.+Woodshttp://search.barnesandnoble.com/booksearch/results.asp?ATH=William+M.+Jandahttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Paul+C.+Schreckenbergerhttp://www.amazon.com/Patrick-R.-Murray/e/B001IGOFSS/ref=ntt_athr_dp_pel_1http://www.amazon.com/Patrick-R.-Murray/e/B001IGOFSS/ref=ntt_athr_dp_pel_pop_1http://www.amazon.com/s/ref=ntt_athr_dp_sr_pop_1?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Patrick%20R.%20Murrayhttp://authorcentral.amazon.com/gp/landing/ref=ntt_atc_dp_pel_1


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Ellen Jo Baron,Michael A. Pfaller, Fred C. Tenover,Robert H. Yolken, editura ASM, 2007

Performance Standards for Antimicrobial Susceptibility Testing.M 100, S19

CLSI /2009

A
http://www.amazon.com/Ellen-Jo-Baron/e/B001HCVG58/ref=ntt_athr_dp_pel_2http://www.amazon.com/s/ref=ntt_athr_dp_sr_3?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Michael%20A.%20Pfallerhttp://www.amazon.com/s/ref=ntt_athr_dp_sr_3?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Michael%20A.%20Pfallerhttp://www.amazon.com/s/ref=ntt_athr_dp_sr_4?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Fred%20C.%20Tenoverhttp://www.amazon.com/s/ref=ntt_athr_dp_sr_5?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Robert%20H.%20Yolkenhttp://www.amazon.com/s/ref=ntt_athr_dp_sr_5?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Robert%20H.%20Yolkenhttp://www.amazon.com/Ellen-Jo-Baron/e/B001HCVG58/ref=ntt_athr_dp_pel_2http://www.amazon.com/s/ref=ntt_athr_dp_sr_3?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Michael%20A.%20Pfallerhttp://www.amazon.com/s/ref=ntt_athr_dp_sr_4?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Fred%20C.%20Tenoverhttp://www.amazon.com/s/ref=ntt_athr_dp_sr_5?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Robert%20H.%20Yolken

Meningococ Si Gonococ Lp UTM

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