Streptococcus Pyogenes Lp UTM

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    Genul Streptococcus spp.

    Streptococi beta hemolitici

    S. pyogenes = Streptococcus grup A;

    S. agalactiae= Streptococcus grup B;Streptococcus grup C;

    Streptococcus grup G;

    etc

    Streptococi grup viridans

    S. pneumoniae (pneumococ)

    S. pyogenes, Streptococcus grup A

    agent etiologic al unor afectiuni grave: infectii respiratorii: faringite, angine;

    Angina eritemato-pultacee

    ANGINA ERITEMATO-P!TA"EE#

    infectii tegumentare: eri$ipel,

    %

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    eri$ipel

    infectii ale tesuturilor moi: fasceite necro$ante, celulite;

    fasceita necro$anta

    &

    http://giulia21.files.wordpress.com/2009/07/fasceita-necrozanta.jpghttp://images.google.ro/imgres?imgurl=http://upload.wikimedia.org/wikipedia/commons/thumb/1/1c/Erysipel.JPG/300px-Erysipel.JPG&imgrefurl=http://bs.wikipedia.org/wiki/Erizipel&usg=__B-g6McAjrLytbuQ6K2MfdMAy5ck=&h=225&w=300&sz=12&hl=ro&start=7&tb%20
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    scarlatin';

    sin(rom (e soc to)ic;

    complicatii supurative prin popagarea infectiei;

    complicatii non-supurative: fe*r' reumatic', glomerulonefrit' acut', coree, eritem no(os

    eritem o(os

    Diagnostic bacteriologic in infectiile produse deS. pyogenes

    Produse patologice:

    secre+ie faringian', secre+ie (in le$iuni cutanate, sange pentru emocultura

    Recoltare produs patologic

    http://www.medspain.com/pautas/enodoso_01.JPGhttp://images.google.com/imgres?imgurl=http://www.anidescoala.ro/images/articles/article_830.jpg&imgrefurl=http://www.anidescoala.ro/Sanatatea-copilului/index1.html&usg=__NaCNUVhXy8-pFPvWeD8DTDUQPLI=&h=100&w=100&sz=5&hl=en&start=20&tbnid=W48vpS%20http://images.google.com/imgres?imgurl=http://www.typesofbacteria.co.uk/images/27433.jpg&imgrefurl=http://www.typesofbacteria.co.uk/what-causes-scarlet-fever.html&usg=__Nw2R7mk0Ri_N_ZbftPWexxQrd74=&h=260&w=200&sz=16&hl=en&start=8&tbnid=SJGmyF7L%20http://giulia21.wordpress.com/2009/07/24/absolut-shocantc3le-my-ciudate-boli-din-lume/omul-pasare/
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    PRODUS

    REO!"#RE

    a)#$"%"#"E

    b)"R#$SPOR"

    O&SER'#"%%

    RESPECTA PRECAUTIUI!EUI"ERSA!E

    E./AT0ARINGIAN

    /eprima *a$a lim*iicu apasator steril#

    1terge cu tamponulferm, nu *rutal,amig(alele,pereteleposterior al farigelui -$one inflamate,ulcerate, (epo$itepurulente#

    a2 tampon cume(iu (e

    transport*2 3&

    4Recoltarea se face inainte (eingestia alimentelor sau toaletacavitatii *ucale# 1e evita atingerea

    tamponului cu *a$a lim*ii sau palatulmoale#tie#

    Eamenul microscopic direct5frotiu din produsului patologic (pp)

    In ca$ul e)su(atului faringian se face frotiu (in pp doar in ca$ul suspiciunii clinice (ecan(i(o$a si suspicinii clinice (e angina 6incent

    %nsamantarea pp

    se insamantea$a in poligon (escis 7 fara o latura2 8 pe me(ii soli(e, in scopul

    o*tinerii coloniilor micro*iene i$olate: gelo$a-sange sau "olum*ia 8sange

    9

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    %ncubare la ", (upa %< 8 &9 ore se face prima citire a placilor, apoi se reincu*ea$a

    pentru inca &9 ore

    %dentificarea bacteriei:

    aractere morfotinctoriale: frotiu din cultur pur : coci Gram po$itivi, =n

    (ispusi =n lanturi

    aractere de cultura - pe gelo$a s>nge: col *S+ cu hemoli,a beta7?EMO!I@A clara, transparenta, totala2 1treptococi

    -emolitici

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    B!oo( agar plate Cit StreptococcuspyogenesstreaDe( at te top an( a troatsCa* on te *ottom# Note te clarit of teclearing aroun( te S. pyogenescolonies,an( te caracteristic greenis tur*i(it ofalpa emolsis in te troat sCa*#

    F

    http://biology.clc.uc.edu/fankhauser/Labs/Microbiology/Strep_Detection/hemolysisP8040182.jpg
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    A mi)e( streaD of non-emoltic 7largercolonies2 an( smaller Streptococcuspyogenescolonies soCing $ones of clearingaroun( eac colon#

    Mi)e( streaD soCing te clearing aroun( aconcentration of S. pyogenescolonies#

    "lose up of a stu(ents troat sCa* Citalpa emolsis surroun(ing te streaD#

    ?ere is a stu(ents culture soCingconsi(era*le *eta emolsis# 1treptococcuspogenes is streaDe( at te top as a positivecontrol#

    "estul catala,ei

    Diferentia,a coloniile destap#ylococde cele de streptococ#

    /eoarece am*ele *acterii cresc pe me(iu cu sange se recoman(a folosirea (oar a

    varfurilor coloniilor (e stafilococ sau streptococ pentru a evita reactii fals po$itive

    (atorita catala$ei eritrocitare7(in me(iu cu gelo$a 8sange2#

    http://biology.clc.uc.edu/fankhauser/Labs/Microbiology/Strep_Detection/throat_culture_P2273129md.JPGhttp://biology.clc.uc.edu/fankhauser/Labs/Microbiology/Strep_Detection/hemolysis_alpha_P7271264.jpghttp://biology.clc.uc.edu/fankhauser/Labs/Microbiology/Strep_Detection/hemolysis_P7271261.jpghttp://biology.clc.uc.edu/fankhauser/Labs/Microbiology/Strep_Detection/hemolysis_P7271262.jpg
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    Pe o lama (e microscop (epune %-& picaturi (e ?&O&,peste picatura (e reactiv se a(auga

    coloniile testate#

    Citire si interpretare:

    Test po$itiv-aparitia *ulelor (e ga$ pana la efervescenta, 1TA0I!O"O"

    Test negativ-a*senta *ulelor, 1TREPTOT"O"

    "este de sensibilitate la bacitracina

    Este aplicat pentru i(entificarea pre$umtiva a 1treptococcus grup A, testul nu pre$inta o

    inalta specificitate (eaorece H% J (intre tulpinile (e 1treptoccus grup " si G, pre$inta

    sensi*ilitate la *acitracina si J (intre 1treptococii (e grup B

    Procedura - se efectuea$' prin repicarea =n p>n$' a coloniei *eta emolitice pe o plac'

    cu "olum*ia-sange# Kn miLlocul $onei se amplasea$' o ron(el' (e Bacitracin'# Placa se

    incu*' peste noapte la " - "# Aparitia unei $one (e ini*itie in Lurul (iscului este

    consi(erat un re$ultat po$itiv, o tulpina sensi*ila la *acitracina

    %dentificarea serologica

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    "estul de late aglutinare pe lama

    se folosesc colonii *eta emolitice (e pe me(iul cu sange

    "lasificarea !ancefile( 8 streptococii sunt clasificati : 1EROgrup A, B, ", / etc

    #ntibiograma

    Streptococcus grup A si-a pastrat sensi*ilitatea la penicilina 8 nu se efectuea$'

    anti*iograma 7numai =n situa+ii speciale, (e e)# alergie la penicilina 2b) Diagnosticul serologic

    testul #S!O (evidentierea #c anti streptoli,ina O)

    1treptoli$ina O 5 1!O 5 Ag (etermina formarea (e #c anti-S!O . #S!O

    A1!O creste =n primele $ile p>n' =n s'ptam>nile -9 c>n( se men+ine =n platou si apoi

    sca(e titrul p>na la valori normale p>na la F luni, (aca s-a intervenit cu un tratament

    corespun$ator 7vin(ecare2;

    sau poate creaste titrul Ac (aca nu a fost tratat *olnavul sau s-a pro(us o complica+ie7RAA, afectare car(iaca, glomerulonefrita renal' acuta, coreea minor etc#2#

    "alori $e re%erin&'

    " (arsta "(aloare )UI*m!+

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    -3%F ani 3%

    H%H%F ani 3&

    /efinitii

    CREE =Boal a sistemului nervos caracterizat de obicei prin micri involuntare i

    necoordonate.

    Modul de desfasurare a lucrarii practice

    studentii primesc manuscrisul cu etapele diagnosticului de laborator in

    infectia cu Streptococcus pyogenes .

    1. Lp se desfasoara interactiv- se discuta pe marginea manuscrisului primit

    anterior LP-ului

    material demonstrativ- lucreaza doar asistentul -testul slidex Strepto Plus

    3. material demonstrativ-medii de cultura-Columbia-sange -colonii de

    S. pyogenes,studentii recunosc coloniile beta hemolitice

    9# Fiecare student prinde imaginea la microscop si examineaza frotiuri :coci

    Gram pozitivi in lanturi

    Bibliografie

    Bacteriologie Medicala, Olga Dorobat, editura UTM, 2006

    Clinical Microbiology Procedures Handbook, Henry D. Isenberg, editata

    American Society for Microbiology,2004

    Koneman's Color Atlas and Textbook of Diagnostic Microbiology,Washington

    C Winn, Stephen D. Allen, Gail L. Woods, William M. Janda, Paul C.

    Schreckenberger, editura Lippincott Williams & Wilkins, 2005

    Manual of Clinical Microbiology , Patrick R. Murray,

    Visit Amazon's Patrick R. Murray Pagesearch results

    Learn about Author Central

    Ellen Jo Baron, Michael A. Pfaller, Fred C. Tenover, Robert H. Yolken, editura

    ASM, 2007

    Performance Standards for Antimicrobial Susceptibility Testing.M 100, S19

    %

    http://shopping.yahoo.com/search;_ylt=AmwIkvVSDn3oMrp235b4BMACyNsA?p=Henry+D.+Isenberghttp://shopping.yahoo.com/search;_ylt=AkzkLWrc9z31QkUKO_a6ltICyNsA?p=American+Society+for+Microbiologyhttp://shopping.yahoo.com/search;_ylt=AkzkLWrc9z31QkUKO_a6ltICyNsA?p=American+Society+for+Microbiologyhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Washington+C+Winnhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Washington+C+Winnhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Washington+C+Winnhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Stephen+D.+Allenhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Gail+L.+Woodshttp://search.barnesandnoble.com/booksearch/results.asp?ATH=William+M.+Jandahttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Paul+C.+Schreckenbergerhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Paul+C.+Schreckenbergerhttp://www.amazon.com/Patrick-R.-Murray/e/B001IGOFSS/ref=ntt_athr_dp_pel_1http://www.amazon.com/Patrick-R.-Murray/e/B001IGOFSS/ref=ntt_athr_dp_pel_pop_1http://www.amazon.com/s/ref=ntt_athr_dp_sr_pop_1?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Patrick%20R.%20Murrayhttp://authorcentral.amazon.com/gp/landing/ref=ntt_atc_dp_pel_1http://www.amazon.com/Ellen-Jo-Baron/e/B001HCVG58/ref=ntt_athr_dp_pel_2http://www.amazon.com/Ellen-Jo-Baron/e/B001HCVG58/ref=ntt_athr_dp_pel_2http://www.amazon.com/s/ref=ntt_athr_dp_sr_3?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Michael%20A.%20Pfallerhttp://www.amazon.com/s/ref=ntt_athr_dp_sr_3?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Michael%20A.%20Pfallerhttp://www.amazon.com/s/ref=ntt_athr_dp_sr_4?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Fred%20C.%20Tenoverhttp://www.amazon.com/s/ref=ntt_athr_dp_sr_4?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Fred%20C.%20Tenoverhttp://www.amazon.com/s/ref=ntt_athr_dp_sr_5?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Robert%20H.%20Yolkenhttp://shopping.yahoo.com/search;_ylt=AmwIkvVSDn3oMrp235b4BMACyNsA?p=Henry+D.+Isenberghttp://shopping.yahoo.com/search;_ylt=AkzkLWrc9z31QkUKO_a6ltICyNsA?p=American+Society+for+Microbiologyhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Washington+C+Winnhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Washington+C+Winnhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Stephen+D.+Allenhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Gail+L.+Woodshttp://search.barnesandnoble.com/booksearch/results.asp?ATH=William+M.+Jandahttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Paul+C.+Schreckenbergerhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Paul+C.+Schreckenbergerhttp://www.amazon.com/Patrick-R.-Murray/e/B001IGOFSS/ref=ntt_athr_dp_pel_1http://www.amazon.com/Patrick-R.-Murray/e/B001IGOFSS/ref=ntt_athr_dp_pel_pop_1http://www.amazon.com/s/ref=ntt_athr_dp_sr_pop_1?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Patrick%20R.%20Murrayhttp://authorcentral.amazon.com/gp/landing/ref=ntt_atc_dp_pel_1http://www.amazon.com/Ellen-Jo-Baron/e/B001HCVG58/ref=ntt_athr_dp_pel_2http://www.amazon.com/s/ref=ntt_athr_dp_sr_3?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Michael%20A.%20Pfallerhttp://www.amazon.com/s/ref=ntt_athr_dp_sr_4?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Fred%20C.%20Tenoverhttp://www.amazon.com/s/ref=ntt_athr_dp_sr_5?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Robert%20H.%20Yolken
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    CLSI /2009

    cvirtuel.cochin.univ-paris5.fr/.../angine.jpg

    http://biology.clc.uc.edu/fankhauser/labs/microbiology/strep_detection/strep_test.htm

    %%