Studierea compartimentelor celulare si a citoscheletului celular pe microfotografii

Vizualizarea organitelor pe microfotografii efectuate la microscopul electronic Clasificarea organitelor

A. Dup funcia lor:1. sintez de proteine Ribozomi2. sortare i transport vezicular molecular n compartimente intracelulare R.E. mol.: RER, REN , Aparatul Golgi , Lizozomi3. Organite implicate n conversia energic i metabolismul celular: Mitocondria (cloroplati) , Paroxizomii, Cloroplast;

B. Dup dimensiunea lor 1. Vizibile n MO mitocondrie 2. Vizibile n ME - aparatul Golgi - RE - cloroplatii - vezicule - ME - ribozomii - centrul celular - matricea celular: MT, MFC. Dup delimitare cu MP 1. delimitate MP M, RE, Ap G., P,L 2. nedelimitate MF, MT, corpi celulari

.Tipuri de adeziune din cellule epiteliale de sobolan:Zonula occludens, Zonula adhaerens si Macula adhaerens. Af = Aktinfilamente din Mikrovilli [Mv] ; reteaua terminal [Rt] Af* = Aktinfilamente, C = Cytoplasma ; Cjc = Complexus junctarum cellularum ; Clv = Vesiculum clathrinum ; Cri = Crista mitochondrialis ; CMV = Corpusculum multivesiculare ; D = Junctio intercellularis digitiformis; Gx = Glykokalix din Zellmembran; IF = Intermedirfilamente; Lyp = primre Lysosomen; Lys = Lysosom secundar; Ma = Macula adhaerens ; Mi = Mitochondrium ; Mm = Membrana mitochondrialis; Mv = Mikrovilli; P = Plasmalemm ; Pr = Polyribosomen ; Rt = Rete terminale Spi = Spatium intercellulare ; Za = Zonula adhaerens (intermediate junction); Zo = Zonula occludens (tight junction );

Junctiuni celulare

Celule epiteliale

C = Cytoplasma, D = Digitatione membranare; Ly = primre Lysosomen; Ma = Macula adhaerens ; Mi = Mitochondrium (Crista-Typ); Mv = Mikrovilli ; P = Plasmalemma ; RER = reticulum endoplasmic rugos; S = Spatium intercellulare .

A = Discus anisotropicus ; Af = Filamentum actinum ; C = Cytoplasma ; Fa = Fascia adhaerens Grg = Granula glycogeni ; H = Zonula luicda ; I = Discus isotropicus (isotrope Zone; If = Filamenta intermedia ; LT = L-Tubulus ; M = Mesophragma ; Ma = Macula adhaerens ; Mc = Macula communicans = Nexus = Gap junction ; Mf = Filamentum myosinium ; Mi = Mitochondrien ; Myf = Myofibrille; Spi = Spatium intercellulare ; tT = T-Tubulus ; Z = Telophragma ; .

Lyzozom

C = Cytoplasma ; Ce = Centriolum ;Golgi-Apparates; Clv = Vesicula clathrina ; Cmv = Corpusculum multivesiculare ; Cri = Crista mitochondrialis ; Ec = Euchromatin; G = Complexus golgiensis (Golgi-Apparat); G* = Golgi-Apparat ; Hc = Heterochromatin; Ko = Kollagenfibrillen ; Lb = Lamina basalis ; Ld = Lamina densa ; Lri = Lamina rara interna ; Lys = Lysosoma secundarium ; Mam = Matrix mitochondrialis ; Mi = Mitochondrien ; Mme = Membrana mitochondrialis externa; Mmi = Membrana mitochondrialis interna; Mne = Membrana nuclearis externa ; Mni = Membrana nuclearis interna ; Mt = Mikrotubulus; MTOC = Mikrotubulus Organisations Zentrum ; P = Plasmalemma ; Pnu = Porus nuclei ; PR = Polyribosomen ; RER = Retikulum rugos endoplasmatic ; SER = Reticul endoplasmic neted ;Sfa = Substantia fundamentalis amorpha ; Spi = Spatium intercellulare ; t = fata trans Golgi-Apparates ; Vs = Vesicula secreta .

Mitocondrie

REN

AG

AG

AG

AG

Peroxizom

Sectiune prinLamina Propria prin limba de maimuta, C, Collagenous Fibers; Ca, capillary; F, Fibroblast; M, Mast Cell; MF, muscle Fiber; N, nerve F F ibroblast; 1,600 X Tesut conjunctiv dens

Tendon . C, Collagenous Fibers; F, Fibroblast. 500 X Figure B. Electron micrograph of a longitudinal section through the same Tendon shown in Figure A. C, Collagen fibrils; F, Fibroblast; N, Nucleus of Fibroblast. 11,400 X Figure C. Electron micrograph of a cross section through the same Tendon photographed in Figures A and B. C, Collagen fibrils; F, Fibroblast; N, Nucleus of Fibroblast; arrow, part of Collagen Fibril cut in longitudinal section. 13,000 X

Splina Figures A and B. Electron micrographs of a reticular Fiber from the red Pulp of the spleen of the macaque cut in cross section. A, amorphous material associated with Collagen fibrils; C, Collagen fibrils; E, Erythrocyte in red Pulp of spleen; L, Lymphocyte; R, cytoplasmic extension of reticular cell; arrow, reticular Fiber; (*) close contact between cell membranes of lymphocvte (L) and reticular cell (R). Figure A, 18,000 X; Figure B, 44,000 X

Fibroblaste ,mastocite si macrofage

Figure A. Fibroblasts in loose connective tissue. C, Collagen fibrils; G, Golgi Complex; N, Nucleus; Nu, Nucleolus; RER, rough Endoplasmic Reticulum; *, peripheral ring of Heterochromatin. 12,000 X Figure B. Mast Cell from the Lamina Propria of the human tongue. CT, connective tissue; G, electron-dense cytoplasmic granules; N, Nucleus of Mast Cell. 7,000 X Figure C. Macrophage engulfing Erythrocyte in monkey spleen. E, Erythrocyte; L, Lysosome; M, Macrophage; N, Nucleus; R, reticular fibers. 9,000 X Figure D. Macrophage engulfing Neutrophil in monkey spleen. M, Macrophage; NP, Neutrophil; PE, pseudopodial extension of Macrophage. 13,500 X

Figures A and B. Matched pair of light and electron micrographs of serial thick and thin sections taken through the anterior (ventral) horn of the spinal cord. C, capillary; D, Dendrite of Motor Neuron; L, Lipofuscin Granules; N, Nucleus; NF, cross section through myelinated nerve Fiber; Ni, Nissl Substance; S, Soma (cell body) of Motor Neuron. 2000 X

Figure A. Light micrograph of the submandibular Ganglion of the rat. C, capillary; CT, connective tissue; M, Mast Cell; N, Nucleus of autonomic Ganglion cell Motor Neuron; NF, nerve fibers; NS, Nissl Substance; Nu, eccentrically placed Nucleolus in Ganglion cell Nucleus; SN, Schwarm cell Nucleus. 2,500 X Figure B. Electron micrograph of serial thin section taken through the same Ganglion cells shown in Figure A. CT, connective tissue; N, Nucleus of autonomic Ganglion cell; NF, nerve fibers; R, free ribosomes; RER, rough endoplasmic reticulum; SN, Schwarm cell Nucleus. 4,000 X

Figures A and B. Matched pair of light and electron micrographs of serial thick and thin cross sections through the skin of the fingertip of the squirrel monkey. DP, dermal papilla; E, duct of Eccrine Sweat Gland; SC, Stratum Corneum; SG, stratum germinativurn; SGr, Stratum Granulosum; SL, Stratum Lucidum; SS, Stratum Spinosum; arrow, surface opening of Eccrine Sweat Gland. 1,900 X

ficat

Ficat

Pancreas

Pancreas

Rinichi

Spermatozoizi

Spermatozoizi