FIŞA DISCIPLINEI
Denumirea disciplinei IMMUNOLOGY
Anul de studiu II Semestrul * I/II Tipul de evaluare finală (E / V / C) E1
Regimul disciplinei (Ob – obligatorie, Op – opţională, F - facultativă) Ob Numărul de credite 6
Total ore din planul de învăţământ 63 Total ore de studiu
individual 100
Total ore pe
semestru 163
Titularul disciplinei Conf. Dr. Petru Cianga
* Dacă disciplina are mai multe semestre de studiu, se completează câte o fişă pentru
fiecare semestru
** C – curs, L – activităţi de laborator, P – proiect
sau lucrări practice
Cunoştinţe minime necesare studenţilor pentru parcurgerea noţiunilor de curs/stagii/lucrări
practice
Cell and molecular biology, biochemistry, genetics, histology, phisiology, histology, microbiology.
Obiectivele disciplinei
Acquiring concepts regarding:
- antigenicity and immunogenicity
- description of the immune system components
- description of immune humoral and cellular responses
- description of molecules within the immunoglobulin superfamily
- description of hypersensitivities
- description of fundamental concepts of autoimmune processes and immunodeficiencies
Programa analitică a cursului Nr. ore
1.Terminology
1.1 Important events in immunology development
1.2 Immunologic memory and vaccination
1.3 Instructive theories
1.4 Selective theories
1.5 General features of innate immunity
1.6 General features of adaptive immunity
1.7 Functionality of the immune system
2 hours
Total C** S L P
63 28 35 Facultatea Medicina
Departamentul Stiinte Morfo-Functionale
Profilul Medicina
Specializarea Medicina Generala
Numărul total de ore (pe semestru) din
planul de învăţământ
63
2.Immunogenicity and antigen structure
2.1 Characteristics of antigenic determinants recognized by antibodies
2.2 Characteristics of antigenic determinants recognized by T cells
2.3 The relationship between an antigenic determinant recognized by a Th
lymphocyte and an antigenic determinant recognized by a B lymphocyte ,
found on a proteic antigenic structure
2.4 Factors and conditions that influence the immunogenicity
2.5 Specificity and cross-reactivity; Clinical applications of cross-reactivity
2 hours
3. Innate immunity – natural barriers, cells and molecules
3.1 Natural barriers: anatomical and physiological elements
3.2 Phagocytosis; Endocytosis; Pinocytosis
3.3 Cells and receptors that promote phagocytosis; the concepts of PAMP
and PRR
3.4 NK cell and non-specific extracellular cytotoxicity
3.5 The eosinophil and its role in immunity
3.6 Macrophages, APCs and their role in antigen processing and presentation
3.7 Mast cells – their physiologic role and their involvement in pathology
3.8 Interferons
3.9 Acute phase proteins
3.10 Receptors of the innate immune system
2 hours
4. Receptors for antigen
4.1 Receptor for antigen of B cell; Immunoglobulins
4.2 Antibody structure
4.3 Immunoglobulinic domain
4.4 Variable regions
4.5 Structure and role of constant regions
4.6 Functional properties of immunoglobulin classes
4.7 Receptor for antigen of T cell;
4.8 TCR αβ; CD3 complex-structure
4.9 Co-receptor molecules CD4 and CD8
4.10 Mechanisms of antigenic recognition of antigen and MHC by T cell
2 hours
5. Genetics of antigen receptor
5.1 Light chain κ genes
5.2 Light chain λ genes
5.3 Heavy chain genes
5.4 Sources of diversity in immunoglobulin generation
5.5 Regulatory mechanisms in immunoglobulin gene expression
5.6 Isotype (class) switch
5.7 TCR gene organization; mechanisms involved in TCR diversity
5.8 Regulation of TCR gene rearrangement
5.9 Secondary type of receptor CD3γδ
2 hours
6. Ontogeny of B and T lymphocyte
6.1 Primary lymphoid organs: thymus and bone marrow
6.2 Ontogeny of T lymphocyte
6.3 Mechanisms that induce self tolerance for T cell lymphocyte
6.4 Subsets of T cells
6.5 Ontogeny of B cell and stromal cell role in B cell ontogeny
6.6 Mechanisms that induce self tolerance in B cell lymphocyte
6.7 CD5+ B cells
6.8 Malignancies that involve B cells
6.9 γδ T lymphocytes
2 hours
7 Major Histocompatibility Complex (HLA)
7.1 Cellular distribution of MHCI and MHCII
7.2 Histocompatibility genetics; sources of diversity in MHC; genic map
7.3 Characteristics and functions of MHC
7.4 HLA complex
7.5 Crystallographic structure of MHC molecules
7.6 Clinical importance of HLA phenotyping
2 hours
8 Antigen processing and presentation
8.1 Antigen presenting cells
8.2 Antigen recognition and immune response
8.3 Identification and characterization of natural peptides associated with
MHCI and MHCII
8.4 Peptides as parts in molecular structure of MHCI and MHCII
8.5 Synthesis and intracellular transport of MHC molecules
8.6 Sites of peptide generation and association with MHC
8.7 General features about pathways of MHC presentation
8.8 Biological consequences of simultaneous recognition MHC-peptide by T
cell
2 hours
9 B and T cell activation
9.1 Experimental study models for T cell activation
9.2 Requirements for Tcell activation initiation; co-stimulatory molecules
9.3 TCR signal transduction
9.4 Consequences of signal transduction; nuclear transcription factors
9.5 T cell proliferation regulation
9.6 T cell inactivation; Anergy induction
9.7 B cell activation
9.8 Signal transduction by mIg
9.9 Early events stimulation in B cell activation
9.10 Antibody response to Ti antigens
9.11 Antibody response to Td antigen
9.12 Antigen presentation to Th cell
9.13 Th cell signals mediated by cell contact
9.14 Regulation of isotype expression
9.15 Production and B memory cells
9.16 B cell activation versus inactivation
2 hours
10 Functions and mechanisms of action of B and T effector cells
10.1 In vivo functions of T citolytic cells
10.2 CTL and NK cells in bacterial, viral and parasitic infections
10.3 CTL and NK cells in tumoral immunity
10.4 NK cells receptors
10.5 CTL’s in autoimmunity
10.6 Interactions lymphocyte-target cell
10.7 Lethal hit and lytic mechanisms induced by lymphocyte
10.8 Auto protective mechanisms of CTL
10.9 Detachment from the conjugate and CTL recycling
10.10 Functional Th populations – balance Th1/Th2
10.11 Memory lymphocytes
10.12 Regulatory lymphocytes
2 hours
11 Cytokines
11.1 The role of cytokines in humoral and cell mediated immunity
11.2 Cytokines’ families
11.3 Cytokines’ structure
11.4 Cytokines’ properties
11.5 Receptor families for cytokines
11.6 Signal transduction through cytokine receptors
11.7 Cross regulation of cytokines
11.8 Cytokines’ antagonists
11.9 Diseases induced though cytokines
2 hours
12. Immune response; Inflammation; Lymphocyte traffic
12.1 Lymphoid organs and tissues – structure, functions
12.2 Lymphocyte recirculation and homing – CD44
12.3 Primary immune response; activation signals
12.4 Germinal center events
12.5 Accessory molecules involved in B-T cell interaction
12.6 Follicular dendritic cells and their involvement in immune response
12.7 Isotype switch and affinity maturation
12.8 Secondary immune response
12.9 Immunological memory
12.10 Adhesion molecules; diapedesis
12.11 Local and systemic acute inflammation; chronic inflammation
2 hours
13. Complement system
13.1 Basal circuit of C3; discrimination self-non self
13.2 Alternative pathway of activation
13.3 Classical pathway; immune complexes role in complement activation
13.4 Membrane attack complex; postC3 pathway
13.5 Receptors for complement
13.6.Anaphylatoxins and receptors for anaphylatoxins; role in inflammation
13.7 Complement system regulation
13.8 The role of complement in infections; inflammatory reaction mediated
by complement
13.9 Acute inflammatory reaction – mediators and effectors
13.10 Deficiencies of some complement system components; clinical
consequences
2 hours
14. Deviated immune response: hipersensitivities; types and general
characteristics
14.1 Hipersensitivity type I – mechanism, role of IgE and mast cells
14.2 Hipersensitivity type I – clinical example
14.3 Hipersensitivity type II- mechanisms of tissue destruction
14.4 Hipersensitivity type II – clinical examples
14.5Hipersensitivity type III – implications in pathology
14.6Hipersensitivity type IV – clinical variants and mechanisms of production
14.7 Hipersensitivity type IV – role of T lymphocytes and cytokines produced
by these cells
2 hours
Programa analitică lucrări practice/stagii
1. Antigenicity/immunogenicity: implications for the immunization process
– 2,5 ore
2,5 hours
2. Cross–reactivity : practical applications 2,5 ore 2,5 hours
3. Classes and subclasses of immunoglobulines – 2,5 ore 2,5 hours
4. Blood groups. ABO and Rh systems. – 2,5 ore 2,5 hours
5. Ag-Ab interaction in agglutination reaction – 2,5 ore 2,5 hours
6. Ag-Ab interaction in precipitation reaction 2,5 hours
7. Hibridoma, monoclonal antibodies 2,5 hours
8. Solid phase tests: RIA, RIST, RAST 2,5 hours
9. Solid phase tests: ELISA – 2,5 ore 2,5 hours
10. Imunohistochemistry, imunofluorescence. 2,5 hours
11. Blot: Western blotting, Southern blotting. 2,5 hours
12. Flow-cytometry 2,5 hours
13. Cellular functionality tests 2,5 hours
14. Immunological investigation in immune pathology 2,5 hours
Competenţe practice dobândite la finalul anului/semestrului - understanding the principles of immunological methods and their application in practice
La stabilirea notei finale se iau în considerare Ponderea în notare exprimată în
procente (Total = 100%)
- răspunsurile la examen / colocviu (evaluarea finală): 50%
- răspunsurile finale la lucrările practice de laborator 35%
- testarea periodică prin lucrări de control 15%
- activităţile gen teme / referate / eseuri / traduceri / proiecte etc.
- alte activităţi (PRECIZAŢI) : colocviu
Descrieţi modalitatea practică de evaluare finală E/V (de exemplu: lucrare scrisă (descriptivă şi / sau test grilă şi / sau probleme etc.), examinare orală, colocviu individual ori în grup, proiect, etc.) - oral examination - MCQ (50 questions)
Estimaţi timpul total (ore pe semestru) al activităţilor de studiu individual pretinse
studentului
(completaţi cu 0 activităţile care nu sunt cerute)
1. Descifrarea şi studiul notiţelor de
curs
14
8. Pregătire prezentări orale 0
2. Studiu după manual, suport de
curs
28 9. Pregătire examinare finală 20
3. Studiul bibliografiei minimale
indicate 24 10. Consultaţii
4. Documentare suplimentară în
bibliotecă 0 11. Documentare pe teren 0
5. Activitate specifică de pregătire
SEMINAR şi / sau LABORATOR 14 12. Documentare pe Internet 0
6. Realizare teme, referate, eseuri,
traduceri, etc. 0 13. Alte activităţi .... 0
7. Pregătire lucrări de control 0 14. Alte activităţi .... 0
TOTAL ore studiu individual (pe semestru) =
minimum 100 ore
- Bibliografie obligatorie: - Tak Mak, Mary E. Saunders: Primer to the Immune Response, 2011, Academic Press,
Elsevier
Bibliografie suplimentară pentru materia de curs şi lucrări practice Imunologie - Richard Goldsby, Thomas J. Kindt, Barbara Osborne : Kuby’s Immunology, 5th
Edition, 2002;
- Charles A. Janeway, Paul Travers, Mark Walport, J. Donald Capra: Immunobiology,
6th Edition, 2004;
- David Male, Jonathan Brostoff, David B. Roth, Ivan Roitt: Immunology, 7th Edition,
2006;
- Abul K. Abbas, Andrew H. Lichtmann: Cellular and Molecular Immunology, 5th
Edition, Updated edition, 2005
Data completării: Semnătura coordonatorului de activitate didactică
2012
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