FIŞA DISCIPLINEI

Denumirea disciplinei IMMUNOLOGY

Anul de studiu II Semestrul * I/II Tipul de evaluare finală (E / V / C) E1

Regimul disciplinei (Ob – obligatorie, Op – opţională, F - facultativă) Ob Numărul de credite 6

Total ore din planul de învăţământ 63 Total ore de studiu

individual 100

Total ore pe

semestru 163

Titularul disciplinei Conf. Dr. Petru Cianga

* Dacă disciplina are mai multe semestre de studiu, se completează câte o fişă pentru

fiecare semestru

** C – curs, L – activităţi de laborator, P – proiect

sau lucrări practice

Cunoştinţe minime necesare studenţilor pentru parcurgerea noţiunilor de curs/stagii/lucrări

practice

Cell and molecular biology, biochemistry, genetics, histology, phisiology, histology, microbiology.

Obiectivele disciplinei

Acquiring concepts regarding:

- antigenicity and immunogenicity

- description of the immune system components

- description of immune humoral and cellular responses

- description of molecules within the immunoglobulin superfamily

- description of hypersensitivities

- description of fundamental concepts of autoimmune processes and immunodeficiencies

Programa analitică a cursului Nr. ore

1.Terminology

1.1 Important events in immunology development

1.2 Immunologic memory and vaccination

1.3 Instructive theories

1.4 Selective theories

1.5 General features of innate immunity

1.6 General features of adaptive immunity

1.7 Functionality of the immune system

2 hours

Total C** S L P

63 28 35 Facultatea Medicina

Departamentul Stiinte Morfo-Functionale

Profilul Medicina

Specializarea Medicina Generala

Numărul total de ore (pe semestru) din

planul de învăţământ

63

2.Immunogenicity and antigen structure

2.1 Characteristics of antigenic determinants recognized by antibodies

2.2 Characteristics of antigenic determinants recognized by T cells

2.3 The relationship between an antigenic determinant recognized by a Th

lymphocyte and an antigenic determinant recognized by a B lymphocyte ,

found on a proteic antigenic structure

2.4 Factors and conditions that influence the immunogenicity

2.5 Specificity and cross-reactivity; Clinical applications of cross-reactivity

2 hours

3. Innate immunity – natural barriers, cells and molecules

3.1 Natural barriers: anatomical and physiological elements

3.2 Phagocytosis; Endocytosis; Pinocytosis

3.3 Cells and receptors that promote phagocytosis; the concepts of PAMP

and PRR

3.4 NK cell and non-specific extracellular cytotoxicity

3.5 The eosinophil and its role in immunity

3.6 Macrophages, APCs and their role in antigen processing and presentation

3.7 Mast cells – their physiologic role and their involvement in pathology

3.8 Interferons

3.9 Acute phase proteins

3.10 Receptors of the innate immune system

2 hours

4. Receptors for antigen

4.1 Receptor for antigen of B cell; Immunoglobulins

4.2 Antibody structure

4.3 Immunoglobulinic domain

4.4 Variable regions

4.5 Structure and role of constant regions

4.6 Functional properties of immunoglobulin classes

4.7 Receptor for antigen of T cell;

4.8 TCR αβ; CD3 complex-structure

4.9 Co-receptor molecules CD4 and CD8

4.10 Mechanisms of antigenic recognition of antigen and MHC by T cell

2 hours

5. Genetics of antigen receptor

5.1 Light chain κ genes

5.2 Light chain λ genes

5.3 Heavy chain genes

5.4 Sources of diversity in immunoglobulin generation

5.5 Regulatory mechanisms in immunoglobulin gene expression

5.6 Isotype (class) switch

5.7 TCR gene organization; mechanisms involved in TCR diversity

5.8 Regulation of TCR gene rearrangement

5.9 Secondary type of receptor CD3γδ

2 hours

6. Ontogeny of B and T lymphocyte

6.1 Primary lymphoid organs: thymus and bone marrow

6.2 Ontogeny of T lymphocyte

6.3 Mechanisms that induce self tolerance for T cell lymphocyte

6.4 Subsets of T cells

6.5 Ontogeny of B cell and stromal cell role in B cell ontogeny

6.6 Mechanisms that induce self tolerance in B cell lymphocyte

6.7 CD5+ B cells

6.8 Malignancies that involve B cells

6.9 γδ T lymphocytes

2 hours

7 Major Histocompatibility Complex (HLA)

7.1 Cellular distribution of MHCI and MHCII

7.2 Histocompatibility genetics; sources of diversity in MHC; genic map

7.3 Characteristics and functions of MHC

7.4 HLA complex

7.5 Crystallographic structure of MHC molecules

7.6 Clinical importance of HLA phenotyping

2 hours

8 Antigen processing and presentation

8.1 Antigen presenting cells

8.2 Antigen recognition and immune response

8.3 Identification and characterization of natural peptides associated with

MHCI and MHCII

8.4 Peptides as parts in molecular structure of MHCI and MHCII

8.5 Synthesis and intracellular transport of MHC molecules

8.6 Sites of peptide generation and association with MHC

8.7 General features about pathways of MHC presentation

8.8 Biological consequences of simultaneous recognition MHC-peptide by T

cell

2 hours

9 B and T cell activation

9.1 Experimental study models for T cell activation

9.2 Requirements for Tcell activation initiation; co-stimulatory molecules

9.3 TCR signal transduction

9.4 Consequences of signal transduction; nuclear transcription factors

9.5 T cell proliferation regulation

9.6 T cell inactivation; Anergy induction

9.7 B cell activation

9.8 Signal transduction by mIg

9.9 Early events stimulation in B cell activation

9.10 Antibody response to Ti antigens

9.11 Antibody response to Td antigen

9.12 Antigen presentation to Th cell

9.13 Th cell signals mediated by cell contact

9.14 Regulation of isotype expression

9.15 Production and B memory cells

9.16 B cell activation versus inactivation

2 hours

10 Functions and mechanisms of action of B and T effector cells

10.1 In vivo functions of T citolytic cells

10.2 CTL and NK cells in bacterial, viral and parasitic infections

10.3 CTL and NK cells in tumoral immunity

10.4 NK cells receptors

10.5 CTL’s in autoimmunity

10.6 Interactions lymphocyte-target cell

10.7 Lethal hit and lytic mechanisms induced by lymphocyte

10.8 Auto protective mechanisms of CTL

10.9 Detachment from the conjugate and CTL recycling

10.10 Functional Th populations – balance Th1/Th2

10.11 Memory lymphocytes

10.12 Regulatory lymphocytes

2 hours

11 Cytokines

11.1 The role of cytokines in humoral and cell mediated immunity

11.2 Cytokines’ families

11.3 Cytokines’ structure

11.4 Cytokines’ properties

11.5 Receptor families for cytokines

11.6 Signal transduction through cytokine receptors

11.7 Cross regulation of cytokines

11.8 Cytokines’ antagonists

11.9 Diseases induced though cytokines

2 hours

12. Immune response; Inflammation; Lymphocyte traffic

12.1 Lymphoid organs and tissues – structure, functions

12.2 Lymphocyte recirculation and homing – CD44

12.3 Primary immune response; activation signals

12.4 Germinal center events

12.5 Accessory molecules involved in B-T cell interaction

12.6 Follicular dendritic cells and their involvement in immune response

12.7 Isotype switch and affinity maturation

12.8 Secondary immune response

12.9 Immunological memory

12.10 Adhesion molecules; diapedesis

12.11 Local and systemic acute inflammation; chronic inflammation

2 hours

13. Complement system

13.1 Basal circuit of C3; discrimination self-non self

13.2 Alternative pathway of activation

13.3 Classical pathway; immune complexes role in complement activation

13.4 Membrane attack complex; postC3 pathway

13.5 Receptors for complement

13.6.Anaphylatoxins and receptors for anaphylatoxins; role in inflammation

13.7 Complement system regulation

13.8 The role of complement in infections; inflammatory reaction mediated

by complement

13.9 Acute inflammatory reaction – mediators and effectors

13.10 Deficiencies of some complement system components; clinical

consequences

2 hours

14. Deviated immune response: hipersensitivities; types and general

characteristics

14.1 Hipersensitivity type I – mechanism, role of IgE and mast cells

14.2 Hipersensitivity type I – clinical example

14.3 Hipersensitivity type II- mechanisms of tissue destruction

14.4 Hipersensitivity type II – clinical examples

14.5Hipersensitivity type III – implications in pathology

14.6Hipersensitivity type IV – clinical variants and mechanisms of production

14.7 Hipersensitivity type IV – role of T lymphocytes and cytokines produced

by these cells

2 hours

Programa analitică lucrări practice/stagii

1. Antigenicity/immunogenicity: implications for the immunization process

– 2,5 ore

2,5 hours

2. Cross–reactivity : practical applications 2,5 ore 2,5 hours

3. Classes and subclasses of immunoglobulines – 2,5 ore 2,5 hours

4. Blood groups. ABO and Rh systems. – 2,5 ore 2,5 hours

5. Ag-Ab interaction in agglutination reaction – 2,5 ore 2,5 hours

6. Ag-Ab interaction in precipitation reaction 2,5 hours

7. Hibridoma, monoclonal antibodies 2,5 hours

8. Solid phase tests: RIA, RIST, RAST 2,5 hours

9. Solid phase tests: ELISA – 2,5 ore 2,5 hours

10. Imunohistochemistry, imunofluorescence. 2,5 hours

11. Blot: Western blotting, Southern blotting. 2,5 hours

12. Flow-cytometry 2,5 hours

13. Cellular functionality tests 2,5 hours

14. Immunological investigation in immune pathology 2,5 hours

Competenţe practice dobândite la finalul anului/semestrului - understanding the principles of immunological methods and their application in practice

La stabilirea notei finale se iau în considerare Ponderea în notare exprimată în

procente (Total = 100%)

- răspunsurile la examen / colocviu (evaluarea finală): 50%

- răspunsurile finale la lucrările practice de laborator 35%

- testarea periodică prin lucrări de control 15%

- activităţile gen teme / referate / eseuri / traduceri / proiecte etc.

- alte activităţi (PRECIZAŢI) : colocviu

Descrieţi modalitatea practică de evaluare finală E/V (de exemplu: lucrare scrisă (descriptivă şi / sau test grilă şi / sau probleme etc.), examinare orală, colocviu individual ori în grup, proiect, etc.) - oral examination - MCQ (50 questions)

Estimaţi timpul total (ore pe semestru) al activităţilor de studiu individual pretinse

studentului

(completaţi cu 0 activităţile care nu sunt cerute)

1. Descifrarea şi studiul notiţelor de

curs

14

8. Pregătire prezentări orale 0

2. Studiu după manual, suport de

curs

28 9. Pregătire examinare finală 20

3. Studiul bibliografiei minimale

indicate 24 10. Consultaţii

4. Documentare suplimentară în

bibliotecă 0 11. Documentare pe teren 0

5. Activitate specifică de pregătire

SEMINAR şi / sau LABORATOR 14 12. Documentare pe Internet 0

6. Realizare teme, referate, eseuri,

traduceri, etc. 0 13. Alte activităţi .... 0

7. Pregătire lucrări de control 0 14. Alte activităţi .... 0

TOTAL ore studiu individual (pe semestru) =

minimum 100 ore

- Bibliografie obligatorie: - Tak Mak, Mary E. Saunders: Primer to the Immune Response, 2011, Academic Press,

Elsevier

Bibliografie suplimentară pentru materia de curs şi lucrări practice Imunologie - Richard Goldsby, Thomas J. Kindt, Barbara Osborne : Kuby’s Immunology, 5th

Edition, 2002;

- Charles A. Janeway, Paul Travers, Mark Walport, J. Donald Capra: Immunobiology,

6th Edition, 2004;

- David Male, Jonathan Brostoff, David B. Roth, Ivan Roitt: Immunology, 7th Edition,

2006;

- Abul K. Abbas, Andrew H. Lichtmann: Cellular and Molecular Immunology, 5th

Edition, Updated edition, 2005

Data completării: Semnătura coordonatorului de activitate didactică

2012