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New Biotechnology Volume 29S September 2012

Keywords:Antibacterial; Lactic acid bacteria; Escherichia coli;

Lactobacillus delbrueckii

http://dx.doi.org/10.1016/j.nbt.2012.08.352

Poster 3.2.18

Influence of boron toxicity on global expression profiles

in wheat (Triticum aestivumL.)

C. Kayihan 1,, M.T. Oz 1, F. Eyidogan 2, M. Yucel 1, H.A. Oktem 1

1Middle East Technical University, Department of Biology, 06800

Ankara, Turkey2Baskent University, Faculty of Education, 06810, Ankara, Turkey

Excess boron (B) is known to negatively affect plant growth.

However, the molecular mechanism of toxicity in plants is not sat-

isfactory elucidated. In this study, global expression differences in

wheat (Triticum aestivumL. cv. Atay-85 and Bolal-2973) leaves and

roots under B toxicity were investigated using DNA microarrays.

Eight day old aseptically grown seedlings were subjected to 5 mM

H3BO3 for 5 days. Total RNAs from leaf or root tissues were isolated

and checked by Bioanalyzer 2100. Expression profiles were deter-mined with Wheat GeneChip. Among 61127 transcripts each

represented with a probe set on the GeneChip fold change of at

least 2 andpvalue of at most 0.05 was considered as an indication

of significant alteration in expression. Compared to unstressed

control (10M H3BO3) conditions, B toxicity resulted in signif-

icant regulation of more than 1000 transcripts in leaf and root

tissues of both cultivars. Furthermore,the numbers of genes signif-

icantly expressed in Atay-85 were higher than those in Bolal-2973.

Transcripts altered under B toxicity included genes coding for tran-

scription factors, peroxidases, ribosomal proteins, and hydrolases

and functioning in biosynthesis of phenylpropanoids. Responsive

genes determined in this study will provide insight into succes-

sive studies on molecular mechanism of tolerance to B toxicity inwheat.

http://dx.doi.org/10.1016/j.nbt.2012.08.353

Poster 3.2.19

Amorfrutins are efficient natural antidiabetics

Christopher Weidner, David Meierhofer, Sascha Sauer

Otto Warburg Laboratory, Max Planck Institute for Molecular Genetics,

Ihnestrae 63-73, 14195 Berlin, Germany

Worldwide increases in the incidence of metabolic diseases call for

alternative strategies for preventing and treating these disorders.

Compounds from edible natural resources provide a structurally

diverse resource to alleviate complex diseases by nutritional inter-

vention. We discovered a family of natural antidiabetics, the

amorfrutins, isolated from two legumes, Glycyrrhiza foetida and

Amorpha fruticosa [1]. The amorfrutins selectively activate the

nuclear receptor PPAR, which results in gene expression andphys-

iological profiles markedly different from activation by known

synthetic PPAR ligands. In metabolic mouse models, amor-

frutins strongly increased insulin sensitivity and improved other

physiological parameters. Using proteome-wide quantitative mass

spectrometry we monitored the treatment of diet-induced obese

mice with the amorfrutins and the diabetes drug rosiglitazone in

adipose tissue, liver and the heart. In contrast to microarray-based

gene expression analysis, we detected striking indication for the

unwanted molecular events of rosiglitazone leading to heartfailure

and other known side effects. The methodology introduced here

can be used for systematic proteome-wide screenings of drug can-

didates and can be adapted further to human patient biopsies. Insummary, we showed that application of diet-derived compounds

may constitute a promisingstrategy to combatcommon metabolic

diseases.

Reference

1. Weidner C, et al.Proc Natl Acad Sci USA 2012;109(May (19)):725762.

http://dx.doi.org/10.1016/j.nbt.2012.08.354

Poster 3.2.20

Liposomes and liposomal nanoemulsions from sunflower

S. Shulga , I. Glukh

Institute for Food Biotechnology and Genomics, National Academy of

Science of Ukraine 2, Osypovskogo str., 04123, Kyiv-123, Ukraine

Sunflower lecithin gives manufactures an attractive alternative

to soy lecithin, without requiring allergen labeling. Sunflower

lecithin is an ideal solution forproducing chocolate, chewing gum,

sauces or instantized foods offering smoothness, fine texture and

good emulsifying properties and drug delivery systems. Use of the

sunflower phosphatide concentrate as source material is one of

promising trends for the commercial use of phosphatidylcholine.

Liposomal technology is readily applied in medicine, cosme-

tology and pharmacology. The experience of encapsulation ofbioactive substances can be useful in food technology.

In the study liposomes were prepared from sunflower lecithin

by some methods. The structure of liposomes and particle size

were analyzed and compared. The possibility of using empty

liposomes as transporting nanosystems was studied. Technology

parameters and conditions for their stability and long-term storage

were investigated as well.

Keywords:Lecithin; Sunflower; Liposomes

http://dx.doi.org/10.1016/j.nbt.2012.08.355

Poster 3.2.21

The effect of heat processing on PCR detection of Genet-ically Modified (GM) soya in meat products

zge zgen Arun, Karlo Muratoglu, Funda Yilmaz

Istanbul University, Veterinary Faculty Food Hygiene, Technology

Department, Turkey

The plantation of GMOs are growing rapidly since they were first

commercialized in 1996. Significant ratio of globally planted soya

is GM. However the public concern over GMOs is growing as

rapidly. Thus most countries and finally Turkish Republic have

released regulations on labeling of GMOs. For this purpose devel-

www.elsevier.com/locate/nbt S127

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New Biotechnology Volume 29S September 2012

oping sensitive methods for detection of GMOs is necessary. PCRis

the most common method used thus far. However processing fac-

tors (low pH,heat, etc.) effects the DNAquality and PCR detection.

Addition of soya to the meat products is common in many coun-

tries and most of them have low pH and heated. The aim of this

study was to evaluate combine effect of heat and pH on PCR detec-

tion of GM soya. Sausage mixtures containing various amounts of

GM soya were prepared, and process simulations were performedrepresenting common pH values and heating temperatures used

for meat products. The effect of heat was inspected by detection

of CaMV 35 S which is a novel sequence present in most GMOs

and lectin which is present in both GM and non GM soya. The

results proved that low amounts of GM soya can still be detected

in processed meat products although low pH and temperature has

an effect on detection limit.

Keywords:GMO; PCR; Soya; DNA

http://dx.doi.org/10.1016/j.nbt.2012.08.356

Poster 3.2.22

Heterologous expression of a barley boron transportergene in tobacco (Nicotiana tabaccum) and analysis of

transgenic plants

Doga Gmsel 1,, M. Tufan Oz 2, Fsun Eyidogan3, Meral Ycel 2,

Yelda zden Ciftci 1, Hseyin Avni ktem 2

1 Gebze Institute of Technology, Department of Molecular Biology,

41400 Kocaeli, Turkey2Middle East Technical University, Department of Biological Sciences,

06800 Ankara, Turkey3Baskent University, Faculty of Education, 06810, Ankara, Turkey

High concentration of boron (B) in soil decreases agricultural yield

in arid and semi-arid regions throughout the world. A proposed

strategy to confer tolerance to B stresses in crops is stable transfor-mation of target plants with genes encoding B tolerance proteins

such as efflux/influx transporters. Therefore in this study, barley B

transporter gene HvBor1awas transferred to the model organism

tobacco via Agrobacterium-mediated transformation to improve

tolerance to B toxicity. Plant transformation vectors pIPKb004

containingHvBor1aand pEarleyGate100 containingHvBor1a-GFP

fusion were transferred to A. tumefaciensstrain C58C1 using elec-

troporation. Leaf disc transformation of tobacco (cv. Samsun and

Petit Havana) and indirect organogenesis were utilized to generate

putative transgenics constitutively expressing HvBor1a. The inte-

gration and expression of gene of interest was confirmed with

molecular analysis. Additionally, B toxicity bioassays were per-

formed to examine the altered phenotype of transgenics and firstgeneration of plants. In conclusion, results of this study suggested

that theHvBor1agene might be of great value in engineering tol-

erance to B toxicity in crop plants.

http://dx.doi.org/10.1016/j.nbt.2012.08.357

Poster 3.2.23

Continuous nisin production ofLactococcus lactisimmo-

bilized with surface displayed chitin binding domain on

chitin

Burcu Krdikanlioglu, mer Simsek

University of Pamukkale, Department of Food Engineering, Denizli,

Turkey

In this study, nisin production ofLactococcus lactisstrains express-

ing surface displayed chitin binding domain (ChBD) wereanalyzed

in a chitin containing continuous fermentation system. In this

respect, two ChBD-displayedL. lactisstrains (PLAC2 and PLAC7),

previously constructed with fusing the chitin binding domain to

C-terminal part(344 or 800 amino acid) of the PrtPofL. lactis, were

used separately. These ChBD-displayed strains were immobilized

to chitin and subsequently included in a continuous fermentation

system with 0.10.9 dilution rates. Nisin production and biomass

concentration dramatically reduced for control strains at high

dilution rates where high nisin and biomass production was main-

tained at both ChBD-displayed L. lactis strains. Maximum nisinproduction at control strains used in a fermentation system with

or without chitin was determined as 2120 IU ml1 where ChBD-

displayedstrains with 344aa or 800aa C-terminal of prtP produced

4920IUml1 and 5520IU ml1 nisin respectively. Besides, nisin

productivityof control strains with or without chitinexistence was

measured as 812IUml1 h and 636IUml1 h respectively. How-

ever, this was measured as 2709IU ml1 h withL. lactisPLAC2 and

4581IUml1 h withL. lactisPLAC7 meaning that nisin productiv-

ity of cells could be improved 7-fold higher. We concluded that

800 aa long C-terminal part of PrtP is more functional and yielded

higher productivity. Also surface displayed chitin binding domain

at nisin producerL. lactiscould be used efficiently in continuous

fermentation system.

Keywords:Lactococcus lactis; Nisin; Chitin binding domain;

Continuous fermentation

http://dx.doi.org/10.1016/j.nbt.2012.08.358

Poster 3.2.24

Production of monoclonal antibody against Staphylo-

coccal Enterotoxin A (SEA)

Arzu Pnarbas 1,, Elif Yolac 1, zlem Ertekin 2, Fatma Ycel 2

1Kocaeli University, Faculty of Medicine, Department of Medical Genet-

ics and Molecular Biology, Umuttepe, Kocaeli, Turkey2 TUBITAK, MAM, Institute for Genetic Engineering and Biotechnology,

Gebze, Kocaeli, Turkey

Staphylococcal Enterotoxins (SEA) cause syndromes like toxic

shock, arthritis, allergic reactions and autoimmune diseases beside

food poisoning. The mortality rate is low for staphylococcal food

poisoning but for children and elderly the mortality rate is in the

range of 0.034.4%. Staphylococcal poisonings causes economical

burden due to loss in the production and medical costs.

SEA is the most common toxic, heat and acid-resistant toxin

among all the SEAs. SEA causes serious contamination around the

S128 www.elsevier.com/locate/nbt