151. g lykeiou-statistiki_proagogikes_2010

International Journal of Biological Macromolecules 72 (2015) 502–509 Contents lists available at ScienceDirect International Journal of Biological Macromolecules j ourna l ho me pa g e: www.elsevier.com/locate/ijbiomac Synthesis of antimicrobial Nisin-phosphorylated soybean protein isolate/poly(l-lactic acid)/ZrO 2 membranes Suwei Jiang a , Hualin Wang a,c,∗ , Chenjiang Chu a , Xingkong Ma a , Min Sun a , Shaotong Jiang b,c a School of Chemistry and Chemical Technology, Hefei University of Technology, Hefei, Anhui 230009, People’s Republic of China b School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009, People’s Republic of China c Anhui Institute of Agro-Products Intensive Processing Technology, Hefei, Anhui 230009, People’s Republic of China a r t i c l e i n f o Article history: Received 4 July 2014 Received in revised form 6 August 2014 Accepted 9 August 2014 Available online 1 September 2014 Keywords: Phosphorylated soybean protein isolate/poly(l-lactic acid)/zirconium dioxide nanofibrous membranes Electrospinning technique Antimicrobial activity and release behavior a b s t r a c t Electrospinning technique was used to fabricate the model drug Nisin loaded phosphorylated soybean protein isolate/poly(l-lactic acid)/zirconium dioxide (Nisin-PSPI/PLLA/ZrO 2 ) nanofibrous membranes. The average diameter of drug carrier PSPI/PLLA/ZrO 2 nanofibers increased with the increase of content PSPI and some spindle-shape beads appeared when PSPI content reached 25 wt%. The loading dosage of Nisin caused no significant changes in the size and morphology of nanofibers when Nisin content was below 9 wt%. There existed hydrogen and Zr–O–C bonds among PSPI, PLLA and ZrO 2 units, and the crystalline of PLLA matrix decreased owning to the introducing of PSPI and ZrO 2 units. Moreover, the water absorption capability and degradation rate of PSPI/PLLA/ZrO 2 nanofibrous membranes increased with increasing PSPI content. The antimicrobial activity and release experimental results showed that Nisin-PSPI/PLLA/ZrO 2 nanofibrous membranes displayed well controlled release and better antimicrobial activity against Staphylococcus aureus (S. aureus), and the Nisin release from the medicated nanofibers could be described by Fickian diffusion model. The Nisin-PSPI/PLLA/ZrO 2 nanofibrous membranes may have potential as a new nanofibrous membrane in drug delivery, food active packaging and wound dressing. © 2014 Elsevier B.V. All rights reserved. 1. Introduction In recent years, many researchers have paid more attention to drug delivery system (DDS) [1,2], which have been developed as a new means of releasing drugs with accurate dosage and convenient administration [3]. It will be promising in clinical application with high effectiveness and safety of drugs [4], and high com- pliance of patients [5]. There has been an explosion of interest in the use of the electrospinning or spraying technique for the delivery of bioactive species [6]. Electrospinning, an easy and ver- satile technique, allows bioactive molecules to be combined with a wide range of additional components and provides a high degree of control over the composition and structure of the produced fibers [6]. Electrospun fibers were widely used in drug delivery [7,8] and wound dressing materials [9,10], attributing to their large ∗ Corresponding author at: School of Chemistry and Chemical Technology, Hefei University of Technology, Hefei, Anhui 230009, People’s Republic of China. Tel.: +86 551 62901450; fax: +86 551 62901450. E-mail address: [email protected] (H. Wang). surface-to-volume ratio, excellent biocompatibility and biodegradability [11–13]. Notably, there has been growing attention to fabricate electrospun fibrous membranes as novel active packaging materials at present [14,15]. Poly(l-lactic acid) (PLLA), a synthetic thermoplastic aliphatic polyester, has been used in various medical applications [16] owning to its excellent biocompatibility and biodegradability, as well as inherent non-toxicity [17]. Soy protein isolate (SPI), a biopolymer extracted from soy bean, has attracted great attention due to its biocompatibility and wide availability [18–20]. Furthermore, SPI modified by phosphorylation (PSPI) can form a large number of hydrogen bonds with molecules structured –OH groups owing to a certain amount of phosphate groups attaching to the phosphorylated protein and enhance the interaction between them. The addition of nanoparticles is convenient to overcome some limita- tions of biopolymers, which can improve the polymers’ properties and extend their application field [21,22]. To our interest, zirconia (ZrO 2 ) nanoparticles can not only improve the thermal stability of polymer but enhance human immunity as a kind of far-infrared material also [23,24]. Zirconia (ZrO 2 ) nanoparticles were success- fully hybridized into PLLA matrix in our previous work [25]. http://dx.doi.org/10.1016/j.ijbiomac.2014.08.041 0141-8130/© 2014 Elsevier B.V. All rights reserved.

151. g lykeiou-statistiki_proagogikes_2010

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