http://www.sciencedirect.com/science/article/pii/003194229400727B

AbstractTwo phenylpropanoid glycosides, a new one, lamalboside (2R-galactosylacteoside) and the known acteoside, the flavonol p-coumaroylglucoside, tiliroside, 5-caffeoylquinic acid (chlorogenic acid), along with rutoside and quercetin and kaempferol 3-O-glucosides were isolated from the flowers of Lamium album.Esters of glycosides were obtained as mixtures of trans/cis isomers. The presence of flavonoid p-coumaroylglucosides links Lamium to other members of the subfamily Lamioideae.

http://www.sciencedirect.com/science/article/pii/003194229183030O

AbstractLamium album contains, besides lamalbid, two new secoiridoid glucosides, alboside A and alboside B, and the known carbocyclic iridoid, caryoptoside, which here is characterized for the first time. Alboside B is an equilibrium mixture of three isomers.

http://www.sciencedirect.com/science/article/pii/S0926669012006073

AbstractThe classic techniques such as: maceration (ME), Soxhlet (SE) and heat reflux extraction (HRE) were compared with modern techniques: ultrasonic extraction (UE), microwave-assisted extraction (MAE) andaccelerated solvent extraction (ASE) for their extraction efficiency of two triterpenic acids from Lamii albi flos. Quantifications of oleanolic and ursolic acid in obtained extracts were performed by HPLC method on a RP-18 column with use of mobile phase consisting of acetonitrile–water–1% phosphoric acid (85:15:0.5, v/v/v), the flow rate was 0.8 mL/min and temperature was 10 °C.MAE in closed system was the most effective technique. The best results for ursolic acid were obtained with use of MAE in closed system for 10 min and 100% of generator power. Oleanolic acid was better extracted with use of milder conditions (30% generator power and 30 min). Ultrasonic assisted extraction proved to be noteworthy, alternative method due to its simplicity, inexpensive equipment and relatively good extraction efficiency.

http://www.sciencedirect.com/science/article/pii/S0378874106004569

Abstract

Methanol, ethyl acetate and heptane extracts of Lamium album L. (Lamiaceae) were tested for the toxicity or ability to stimulate growth of human skin fibroblasts (HSF) in vitro. The biological effect depended on the HSF cells density and on the kind and concentration of extract that was used. At a density of 1 × 105 HSF cells/mL no cytotoxicity of extracts was observed during 24 h of incubation. During 24–72 h of incubation with a low (2 × 104 HSF cells/mL) density of cells, significant cytotoxicity was observed for methanol and ethyl acetate extracts at concentrations greater than 125 μg/mL. At concentration of 25 μg/mL the cells remained intact. However, when the cells were incubated with the heptane extract, there was observed relative high viability (>60%) of cells and significant, gradually increasing in time, cellular mitochondrial dehydrogenase activity, measured by MTT assay. Stimulation of human skin fibroblasts proliferation by the heptane extract of Lamii albi flos may indicate for its usefulness of wound healing in vivoand probability of perspectives its use in designing new medicinal plant preparations.In order to determine the biologically active compounds, the plant extracts were separated by high performance thin layer chromatography (HPTLC) on silica gel Si 60 F254 combined with densitometry. Phenolic acids were investigated by high performance liquid chromatography (HPLC).

http://www.sciencedirect.com/science/article/pii/S0367326X06000815

AbstractAntioxidative effects of methanolic extracts from six wild European Lamiaceae species have been studied with the use of three in vitro assays. The ability of scavenging free radicals was measured by DPPH reduction spectrophotometric assay. The reducing potential towards transition metals was tested by phosphomolybdenum method and the inhibition of lipid oxidation was tested by Fe/ascorbate system with photometric TBARS detection. All studied herbs exposed strong antioxidant capability, but the results were different for each species depending on the applied test. In DPPH scavenging the order from strongest to the weakest was: Leonurus cardiaca, Lamium album, Marrubium vulgare, Stachys officinalis, Lamium purpureum, Galeopsis speciosa. With phosphomolybdenum method the extract of S. officinalis was the strongest in both 40 °C and 90 °C but other species acted differently in both temperatures. In lipid peroxidation assay, the maximum inhibition of 78% was reached by S. officinalis and M. vulgare, whereas for both Lamium sp. and L. cardiaca slightly exceeded 70% and for G. speciosa reached 65%. The observed differences indicate the complexity of involved mechanisms and support the necessity of combining several assays in studying the antioxidant potential of medicinal plants.

http://www.sciencedirect.com/science/article/pii/S0378874105001662

Abstract

Research was done on the presence of enzymes in juice obtained from fresh plant material from Chamomilla recutita L. (Rauschel)-anthodium, Lamium album L.-flos, Calendula officinalis L.-flos, Plantaginis lanceolataL.-folium and Euphrasiae rostkoviana Hayne-herba, and in the prepared water infusion of these materials; the objective was to determine the activity of enzymes which beside biologically active substances may have an influence of the final therapeutic effect of the applied plant preparations. The research was conducted by means of the API ZYM system (bioMèrieux). Higher enzymatic activities were found in fresh juices of the examined plant material than in prepared water infusions from dried plants. In both cases naphthol-AS-BI-phosphohydrolase should have highest activity. The second one in terms of activity out of 17 studied enzymes was acidic phosphatase. The highest enzymatic activity of fresh juice was found in Lamii albi flos and Calendulae officinalis flos. Water infusions showed the highest enzymatic activity in Lamii albi flos, Chamomille recutita anthodium and Plantaginis lanceolata folium. Drying the plant material resulted in decreased enzymatic activities but not in the case of naphthol-AS-BI-phosphohydrolase and acidic phosphatase which showed very low activities. The complex composition of plant materials in terms of content of biologically active substances may imply that the therapeutic effect might be directly related to the quantity and activity of plant enzymes present in preparations applied in therapeutics.