8/12/2019 Poster Scoala de Vara 2013 Debrecenjhgggl

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The study of physical and chemical compatibility of izohydrafural,

methyluracil and benzocaine by infrared spectroscopyGorodetchi Ecaterina, Donici Elena, Donici Igor, Uncu Livia

State University of Medicine and Pharmacy Nicolae Testemitanu of the Republic of Moldova

Department of Pharmaceutical and toxicological chemistry

he infrared spectra of pure and mixed substances were recorded between 400 and 7000 cm -1powder, at room temperature.

the spectrum of izohydrafural, the broad bands at 2930 cm-1and 2813,65 cm-1correspond to

e aromatic ring, the absorption band of carbonyl group C=O stretching secondary amide at

82 cm-1. The peaks at 1271 and 961 cm-1belong to organic nitro group. Te peak observed at

9 cm-1 is characteristic for valences oscillations of C-N-O structural unit.

The purpose is to research the physical and chemical

compatibility of izohydrafural, methyluracil and

benzocaine by IR spectrophotometric method. This study

will prove the possibility of a combination of

izohydrafural, methyluracil and benzocaine in the same

dosage form.

High antibacterial

activity;

Rapid antibacterial

action;

The lack of

exciting,carcinogenic

and embryotoxic sideeffects;

6-7 times lower

toxicity than

nitrofural(furacilina).

RESULTS

Stimulates cellular

regeneration and

leucopoieza processes;

Has anti-inflammatory

properties;

Stimulates phagocytic

reaction;For external use treats

wounds, fractures and

burns.

It was researched the physical and chemical compatibility of izohydrafural, methyluracil

and benzocaine by IR spectrophotometric method. The results of this study allow the

combination of izohydrafural, methyluracil and benzocaine in the same dosage form.

Active substances: Izohydrafural, Methyluracil, Benzocaine;FT-IR Bruker Equinox 55 spectrometer

MATERIALS AND METHODSINTRODUCTION

Why Izohydrafural ,

Methyluracil and Benzocaine?

PURPOSE AND AIMS

CONCLUSIONS

The qualitative aspects of infrared spectroscopy are

one of the most powerful attributes of this diverse

analytical technique. IR spectrophotometric method

is a modern method, which is used to identify the

molecular structure of the drug substances based on

the spectra, resulting from the interaction of light

with certain functional groups, bonds and structural

units. IR spectrophotometric method was used in this

study to determine the physical and chemical

compatibility of izohydrafural, methyluracil and

benzocaine by interpretation of the spectra of pure

and mixed substances.

High analgesic

and action;

Topical anesthetic

action;

It works by

blocking nerve

signals in yourbody;

helps to numb the

pain and itching.

In the spectrum of methyluracil, the coupled stretching vibrations of NH from -NH2group isat 3088 cm-1, the band at 1705 cm-1corresponds to the carbonyl group C=O. The methyl group

-CH3is evidenced by the band recorded due to asymmetrical valency vibration (stretching) at

2800 and 2845 cm-1and absorption band in the region of strongest vibration 1347 cm-1. The

peak at 1513 cm-1belongs to vibrations of N-H.

the spectrum of benzocaine, the peak at 3419 belongs to aromatic amino group NH2.The

nd at 2984 cm-1corresponds to strong C-H bonds. The carbonyl group C=O from the ester

oup is evidenced by the band at 1638.

The spectrum of a mixture of izohydrafural, methyluracil and benzocaine has the most

characteristic absorption bands of each substance, which indicates the physical and chemical

compatibility of the molecules of the substances.

O NO2

N CH=N NH C

O

g. 1. Absorption spectrum of Izohidrafural Fig. 2. Absorption spectrum of Methyluracil

ig. 3. Absorption spectrum of Benzocaine Fig. 4. Absorption spectrum of a mixture of izohydrafural, methyluracil and benzocaine.

Biopharmaceutical parameters:Izohydrafural is yellow or greenish-yellow powder with a bitter taste. Very slightly soluble in

water, slightly soluble - in alcohol, soluble in alkalis. Solubility in water at 25 C = 210 mg/l.

Ttop.= 238

Methyluracil is the white crystallinepowder and odourless. Little is soluble in water (up to

0,9% at a temperature of + 200C) and alcohol. Mr = 126,12 Da

Ttop.= 275 pKa = 9,7

Benzocaine is White cristalline powder, odouless. Sparingly soluble in water; it is more soluble

in dilute acids and very soluble in ethanol, chloroform and ethyl ether.

Mr = 165,189 Da. pKa = 2,5