digestie si absorbtie

Grsimi Hidrati de carbon Proteine

Acizi grasi + Glicerol Glucoz Aminoacizi

-oxidare ATP Acetil-CoACiclul Krebs

O2

2H

Mitocondrie

H2O Lantul respirator ADP

Surse extramitocondriale de echivalenti reductori

Spatiu intermembranar Translocarea acizilor monocarboxilici Translocarea acizilor dicarboxilici Translocarea acizilor tricarboxilici Translocarea fosfatului Translocarea nucleotidelor cu adenin Translocarea aspartat - glutamat Translocarea malat - - cetoglutarat PiruvatHPO42Malat2H2PO4ADP3Glutamat

Membrana intern

MatrixHO-

Malat2Citrat3- + H+ H+ Mersalil Atractilozid

ATP4Aspartat

Malat

-cetoglutarat

Proteine cu fier i sulf

(Cys)-S (Cys)-S Fe (Cys)-S S S Fe S-(Cys) (Cys)-S S S-(Cys) Fe S

S Fe

S-(Cys)

Fe S Fe S-(Cys)

Centru binuclear Fe2S2Cys4

Centru tetranuclear Fe4S4Cys4

O H3CO CH3 +H ; e H3CO O Ubichinon (forma oxidat) R= CH2 CH+ -

O H3CO

.CH3 +H ; e+ -

OH H3CO CH3

+ R -H ; e

H3CO

R

-H+ ; e-

H3CO

R OH Ubichinol (forma redus)

CH3 C CH2

OH Semichinon (forma radicalic)n

H

Citocromul cMet 80CH 3 H 3C CH 2 CH N N CH 2 O OC-

S

CH 2

H 3C

HC

CH 3

Fe2+N

N

CH 2

HC CH 2

CH CH 3

CH 2 CH 3

S

CH 2

O OC

-

CH 2

Hys 18

Lant polipeptidic

Organizarea componenilor lanului transportorilor de electroni- 0,32 V + 0,04 V + 0,25 V + 0,82 V

NADH

FPN

CoQ FPS

cit. b

cit. c1

cit. c

cit. a-a3

O2

Succinat

SuccinatComplex II FPS (FAD) (Succinat - CoQ reductaz) Fe-S

NADH + H+

FPN (FMN) Fe - S Complex I (NADH - CoQ reductaz)

CoQ

cit.bK, bT, c1 Fe - S Complex III CoQH2 - cit. c reductaz

cit. c

cit. a-a3 Cu Complex IV Citocrom c oxidaz

O2

Complexul I (NADH-CoQ reductaza)CoQNADH+H NAD++

FPN(FMN) FPN(FMNH2)

1e- FeS 1e 1 2H+

-

FeS2

1e-

FeS3

1e-

FeS4

2e2H+ CoQH2

COMPLEXUL II (SUCCINAT Coq REDUCTAZA)

Succinat

FPs(FAD)

CoQH2

Fumarat

FPs(FADH2)

CoQ

Complexul III (CoQH2-citocrom c reductaza)CoQ-citocromc reductaz CoQH2 2 cit.c (Fe3 ) CoQ 2 cit.c (Fe2 ) 2H

COMPLEXUL IV (CITOCROM C OXIDAZA)

O2 + 4 e + 4 H

-

+

Citocromoxidaz

2 H2O

ADP + Pa

ATP

nH+ Spatiu intermembranar Membrana intern mitocondrialFMN

nH+ + + + CoQ Fe-SFe-S

nH+ +Cit.c Cit.a-a3 (Cu)

+Fe-S

+

Cit. b

2eFADH2

Cit.c1 - -

NADH (2e-) NAD+

Succinat Fumarat

Oxaloacetat Malat Matrix mitocondrial

1/2O2 H2O ATP H+ sintaza

-

+ + + + +

ADP + Pi ATP ATP

ATP-ADP antiport

ADP